TRANSACTIONS OF THE INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGIES
Institute of Molecular Biology, Ministry of Science and Education of the Republic of Azerbaijan
TRANSACTIONS OF THE INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGIES
Institute of Molecular Biology, Ministry of Science and Education of the Republic of Azerbaijan
*For correspondence: musa.karadag@igdir.edu.tr
Musa Karadağ: https://orcid.org/0000-0003-2498-3403 Zübeyir Güneş: https://orcid.org/0000-0002-9170-7006 Kadir Sinan Aslan: https://orcid.org/0000-0003-4564-1285 Halil İbrahim Ünsal: https://orcid.org/0009-0006-6791-3754 Necmettin Aktepe: https://orcid.org/0000-0003-2192-9049 Beşir Dağ: https://orcid.org/0000-0002-5208-3552
The present research aimed to characterize the phenolic composition of eucalyptus (Eucalyptus spp.) leaf extract and to investigate the possible industrial applications of these bioactive constituents. Phenolic compounds were identified and quantified through high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). Analytical findings demonstrated the presence of nearly twenty distinct phenolic substances within the extract. Among the detected compounds, alizarin exhibited the highest concentration (265.72 ng/µL), followed by gallic acid (86.73 ng/µL). Several additional phytochemicals, including ascorbic acid, hydroxybenzoic acid, rutin, naringin, hesperidin, resveratrol, and quercetin, were also detected at varying levels. The diverse phenolic profile obtained from eucalyptus leaves suggests a substantial reservoir of biologically active molecules with notable functional properties. Many of these compounds are recognized for their capacity to neutralize reactive oxygen species, suppress microbial growth, and modulate inflammatory processes. Consequently, eucalyptus-derived extracts may represent promising natural ingredients for incorporation into pharmaceutical preparations, cosmetic products, and health-related formulations. Moreover, their potential role as naturally derived preservatives and oxidation inhibitors may support the development of cleaner-label food products and reduce dependence on synthetic additives. Overall, the findings highlight eucalyptus leaves as a valuable source of phenolic constituents with broad applicability in natural product research. The observed phytochemical richness further supports the utilization of eucalyptus biomass in environmentally friendly production systems, sustainable biotechnology, and green chemistry-based innovation strategies aimed at developing high-value bioactive products.
The authors would like to thank all individuals and institutions that provided support or insights during the preparation of this study.
This study complies with internationally accepted research ethics standards. No human participants, personal data, or confidential information were involved, or all ethical approvals were obtained where required.
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
The authors declare no conflict of interest related to this study.
Aras A., Karadağ M. (2024) Analysis of secondary metabolite contents of some plants by HPLC. Journal of Agriculture, 7(2): 157–167.
Bakkali F., Averbeck S., Averbeck D., Idaomar M. (2008) Biological effects of essential oils – A review. Food and Chemical Toxicology, 46(2): 446–475.
Başar Y., Gül F., Karadağ M., Alma M.H., Demirtas İ., Tel A.Z. (2024) Determination of the volatile, phenolic and fatty acid contents of Helichrysum plicatum by chromatographic methods. Turkish Journal of Biodiversity, 7(2): 83–94.
Dhakad A.K., Pandey V.V., Beg S., Rawat J.M., Singh A. (2018) Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review. Journal of the Science of Food and Agriculture, 98(3): 833–848.
Draelos Z.D. (2019) The science behind skin care: Moisturizers. Journal of Cosmetic Dermatology, 18(4): 1136–1141.
Ekor M. (2014) The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4: 177.
FAO (2021) The State of the World's Forests 2020: Forests, biodiversity and people. Food and Agriculture Organization of the United Nations.
Karadag M., Doğan S. (2024) Mask for moisturizing skin and body made from cold-pressed paste of peanuts (Arachis hypogaea L.). Advances in Biology & Earth Sciences, 9(1): 155–160.
Karadağ M., Omarova S. (2024) Use of Prunus armeniaca L. seed oil and pulp in health and cosmetic products. Advances in Biology & Earth Sciences, 9(1): 105–110.
Karadag M., Baran A., Güneş Z. (2024) Production of value-added cosmetic products from cold-pressed Helianthus annuus L. oil and pulp. Advances in Biology & Earth Sciences, 9: 35–39.
Karadağ M. (2025) Phenolic compound profile of Mentha arvensis L. (wild peppermint) samples. Turkish Journal of Technical Sciences and Innovation, 1(1): 12–23.
Karadağ M., Güneş Z., Çelik S. (2025) Chemical composition of essential oil obtained from rosemary (Rosmarinus officinalis L.) growing in Iğdır and its use in cosmetics. Journal of Agriculture, 8(1): 53–64.
Karadağ M., Koyuncu M., Atalar M.N., Aras A. (2021) SPME/GC-MS analysis of Artemisia campestris subsp. glutinosa, Lavandula angustifolia Mill., and Zingiber officinale volatiles. Erzincan University Journal of Science Institute, 14: 41–49.
Koçak M.Z., Karadağ M., Çelikcan F. (2021) Essential oil composition of Salvia officinalis and Rosmarinus officinalis. Journal of Agriculture, 4(1): 39–47.
Kumar A., Ilavarasan R., Jayachandran T., Decaraman M., Aravindhan P., Padmanabhan N., Krishnan M.R.V. (2012) Phytochemical investigation on a tropical plant Eucalyptus globulus (L.). International Journal of Pharmaceutical Sciences Reviews and Research, 6(2): 42–45.
Li Y., Yao J., Han C., Yang J., Chaudhry M.T., Wang S., Liu H., Yin Y. (2020) Quercetin, inflammation and immunity. Nutrients, 8(3): 167.
Mokhtari Z., Baluchnejadmojarad T., Roghani M. (2021) Neuroprotective effect of gallic acid in a rat model of diabetic neuropathy: Involvement of oxidative stress and inflammation. Iranian Journal of Basic Medical Sciences, 24(2): 180–187.
Silva J., Abebe W., Sousa S.M., Duarte V.G., Machado M.I., Matos F.J. (2003) Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. Journal of Ethnopharmacology, 89(2–3): 277–283.
Suh D.H., Lee S., Heo J. (2019) Ascorbic acid and its antioxidant function in plants. Antioxidants, 8(6): 180.
Yoon S., Park Y., Kim S. (2020) Antioxidant and anti-inflammatory properties of alizarin isolated from Rubia cordifolia. Pharmaceutical Biology, 58(1): 715–722.
Zhang N., Baran A., Valioglu F., Teng L., Atalar M.N., Keskin C., Beilerli A. (2023) Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract. Cellular and Molecular Biology, 69(14): 211–216.
Received: May 12, 2026; Received in revised form: June 12, 2026; Accepted: June 20, 2026
DOI:
https://doi.org/10.62088/timbb/10.1.10Keywords:
Eucalyptus spp., phenolic Compounds, HPLC-DAD, cosmetics, healthcare sector
Karadağ M., Aslan K., Güneş Z., Ünsal H., Aktepe N., Dağ B. (2026). Utilization of eucalyptus (Eucalyptus spp.) plant in value-added cosmetic, health and food products: Biological activities, industrial potential and economic importance . TIMBB, 10 (1), 94-104.