TRANSACTIONS OF THE INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGIES
Institute of Molecular Biology, Ministry of Science and Education of the Republic of Azerbaijan
* For correspondence: minare.hasanova@inbox.ru
Gulnar Aidarkhanova: https://orcid.org/0000-0002-5108-8036 Arzu Çığ: https://orcid.org/0000-0002-2142-5986 Elman Iskender: https://orcid.org/0000-0001-5222-9366 Minara Hasanova: https://orcid.org/0000-0001-5222-9366 Zumrud Mamedova: https://orcid.org/0000-0001-5116-0520 Gullu Aliyeva: https://orcid.org/0000-0001-8020-6583
Given the widespread popularity of common juniper in folk and traditional medicine, as well as in landscape design, this study aimed to investigate the phytochemical composition of Juniperus communis L. growing within the territory of the Burabay State National Nature Park in Northern Kazakhstan to determine its pharmacological potential, and to conduct a comparative study of the biochemical components of Juniperus communis L. and Juniperus sabina L. collected from the study areas Khizi, Altiagaj and Shabran, in Azerbaijan. The distribution and geobotanical characteristics of Juniperus communis L., growing in the Burabay State National Nature Park in northern Kazakhstan, were studied. The phytochemical composition of alcoholic extracts of common juniper included 54 components belonging to 15 classes of organic compounds: Ester, Carboxylic acid, Hydrocarbon, Lactone, Pyranone, Diketone, Ketone, Oxime, Phenol, Alcohol, Epoxide, Ether, Aldehyde, Disaccharide, and Anhydrosugar. Essential oils of 3 juniper species were obtained and their yield percentage was calculated. The yield of essential oil for Juniperus communis L. – 1.4% and and Juniperus sabina – 1.2%. The highest yield of essential oil was recorded in the type of multi-fruited juniper. The article presents the results of the analysis of the essential oil of Juniperus communis L. by gas chromatography and the composition of the essential oil. Chromatographic analysis revealed that thiujone (35.21%) and alpha-terpinene (19.78%) components had higher concentrations, while camphor (0.33%) and estragole (0.16%) components had the lowest concentrations. Turkish scientists have scientifically proven that the biochemical composition of juniper species growing in natural conditions depends on environmental conditions and soil characteristics, especially their richness in potassium (5.02-6.81%), and their macro and micro element composition.
The authors express their sincere gratitude to V.Yu.Kirillov for consultation in interpreting the chemical analysis results and to A.N.Ebel for assistance with the expedition work.
---
The authors have carefully respected ethical standards related to the use of traditional knowledge by appropriately acknowledging all sources and avoiding misrepresentation or misuse of indigenous ethnobotanical information.
This study was carried out within the framework of Project AP05136154 “Resource Potential of Non-Wood Forest Materials and Their Environmental Safety for the Socio-Economic Development of the Regions of Kazakhstan”, financed by the Ministry of Education and Science of the Republic of Kazakhstan during 2018-2020.
There are no conflicts of interest to declare.
Adams R.P., Beauchamp P.S., Dev V., Bathala R.M. (2010) The leaf essential oils of Juniperus communis L. varieties in North America and the NMR and MS data for Isoabienol. Journal of Essential Oil Research, 22(23): 23–28. doi:10.1080/10412905.2010.9700258.
Aleshina E.N., Velichko N.A. (2004) Research of the chemical composition features of essential oils of two Juniperus species L. Chem. Plant Raw Mat., 4: 35-37 (in Russian).
Asgary S., Naderi G.A., Shams Ardekani M.R., Sahebkar A., Airin A., Aslani S., Kasher T., Emami S.A. (2014) Inhibition of protein glycation by essential oils of branchlets and fruits of Juniperus communis subsp. hemisphaerica. Res. Pharm. Sci., 9(3): 179-185; PMID: 25657787.
Butkiene R., Nivinskiene O., Mockufc D. (2009) Two chemotypes of essential oils produced by the same Juniperus communis L. growing wild in Lithuania. Chemija, 20(3): 195-201.
Bykov B.A. (1970) Introduction to phytocenology. Alma-Ata: ANKazSSR Publishing House, 226 p. (in Russian).
Elsherif K.M., Sulaiman M.A., Mlitan A. (2024) Chemical composition and phytochemical screening of Juniperus phoenicea L.: Evaluation of antioxidant activity, minerals, and bioactive compounds. Journal of the Turkish Chemical Society Section A: Chemistry, 11(2): 709-722; doi:10.18596/jotcsa.1330273.
Eryigit T., Yildirim B., Ekici K. (2023) Chemical composition, antioxidant and antibacterial properties of Juniperus excelsa M. Bieb. leaves from Türkiye. Acta Scientiarum Polonorum: Hortorum Cultus, 22(1); doi:10.24326/asphc.2023.4577.
Fatima T., Shahzad M.I., Shah A.N., Yi Z., Gillani B., Hussain I., Gillani A., Çiftçi Z., Locatelli M., Hatamleh A.A., Al-Dosary M. (2025) Phytochemical analysis and in vitro and in vivo pharmacological activities of Cordia myxa extracts. Turkish Journal of Agriculture and Forestry, 49(1): 169-181; doi:10.55730/1300-011X.3256.
Flora of Kazakhstan (1956) Alma-Ata: SciA of Kaz SSR, V.1: 71 pp. (in Russian).
Gerling N.V., Punegov V.V., Gruzdev I.V. (2016) Component composition of essential oil of common juniper (Juniperus communis L.) under the canopy of spruce forests in the European North-East Russia. Chem. Plant Raw Mat., 2: 89-96; doi:10.14258/jcprm.2016021028.
Gülser F., Çığ A., Türkoğlu N. (2012) The determination of nutrient contents of native juniper (Juniperus excelsa Bieb.) and soil properties of plant growing areas in Van. Journal of the Institute of Science and Technology, 2(2): 93-98.
Jegal J., Chung K.W., Chung H.Y., Jeong E.J., Yang M.H. (2017) The standardized extract of Juniperus communis alleviates hyper-pigmentation in vivo HRM-2 hairless mice and in vitro murine B16 melanoma cells. Biol. Pharm. Bull., 40(9): 1381-1388; doi:10.1248/bpb.b17-00122.
Jegal J., Park S.A., Chung K., Chung H.Y., Lee J., Jeong E.J., Kim K.H., Yang M.H. (2016) Tyrosinase inhibitory flavonoid from Juniperus communis fruits. Biosci. Biotechnol. Biochem., 80(12): 2311-2317; doi:10.1080/09168451.2016.1217146.
Karomatov I.D., Davlatova M.S. (2018) Juniper in traditional and scientific medicine. Biology and Integrative Medicine, 1(18): 87-106 (in Russian).
Khan M., Khan A.U., Najeeb-ur-Rehman, Gilani A.H. (2012) Pharma-ecological explanation for the medicinal use of Juniperus excelsa in hyperactive gastrointestinal and respiratory disorders. J. Nat. Med., 66(2): 292-301; doi:10.1007/s11418-011-0605-z.
Koenig W.A., Joulain D., Hochmuth D.H. (2004) Terpenoids and related constituents of essential oils. Mass Finder 3 in convenient and rapid analysis of GC/MS. Hamburg: Hochmuth Scientific Consulting, 493 p.
Kupriyanov A.N. (2020) Conspectus of flora of Kazakh hillocky area. Novosibirsk: Geo Publishing, 358 pp. (in Russian).
Miceli N., Trovato A., Dugo P., Cacciola F., Donato P., Marino A., Bellinghieri V., La Barbera T.M., Güvenç A., Taviano M.F. (2009) Comparative analysis of flavonoid profile, antioxidant and antimicrobial activity of the berries of Juniperus communis L. var. communis and var. saxatilis Pall. Journal of Agricultural and Food Chemistry, 57(15): 6570-6577; doi:10.1021/jf9012295.
Novikov O.O., Pisarev D.I., Zhilyakova E.T., Trifonov B.V., Levchenko V.E., Kornienko I.V. (2013) General knowledge and state of research in pharmacology of the genus Juniperus L. plants. Scientific Bulletin of Belgorod State University, 24(1): 5-9.
Oztürk M., Tümen I., Uğur A., Aydoğmuş-Öztürk F., Topçu G. (2011) Evaluation of fruit extracts of six Turkish Juniperus species for antioxidant, anticholinesterase and antimicrobial activities. J. Sci. Food Agric., 91(5): 867-876; doi:10.1002/jsfa.4258.
Rabotnov T.A. (1964) Determination of the age composition of species populations in a community. Field geobotany. Moscow-Leningrad: Academy of Sciences of the USSR, pp. 132-145.
Salman M., Abdel Hamed E.S.S., Bazaid A.S., Dabi M.M. (2015) Chemical composition for hydrodistillation essential oil of Mentha longifolia by GC-MS from Saudi Arabia. Der Pharma Chimica, 7: 34-40.
Specially protected natural areas. URL: https://gis-terra.kz/spisok-gosudarstvennyh-osobo-ohra (in Russian).
Taviano M.F., Marino A., Trovato A., Bellinghieri V., La Barbera T.M., Güvenç A., Miceli N. (2013) Juniperus oxycedrus L. subsp. oxycedrus and subsp. macrocarpa: Comparative evaluation of phenolic profile, antioxidant, cytotoxic and antimicrobial activities. Food and Chemical Toxicology, 58: 22-29.
Tkachev A.R. (2008) Study of plant volatiles. Novosibirsk, 969 pp. (in Russian).
Türkoğlu N., Balta M.F., Cığ A. (2008) Sugar contents of juniper plants. Asian Journal of Chemistry, 20(4): 3307-3309.
Zhao J., Liu T., Xu F., You S., Xu F., Li C., Gu Z. (2016) Anti-arthritic effects of total flavonoids from Juniperus sabina on arthritis in rats. Pharmacognosy Magazine, 12(47): 178-183; doi:10.4103/0973-1296.186346.
Received: April 18, 2026; Reviewed: June 02, 2026; Accepted: June 12, 2026
DOI:
https://doi.org/10.62088/timbb/10.1.6Keywords:
Juniperus communis L., biochemical components, component composition chromatogram
Aidarkhanova G., Çığ A., Iskender E., Hasanova M., Mamedova Z., Aliyeva G. (2026). Biochemical components of Juniperus communis L. under the conditions of Northern Kazakhstan and the study of essential oils of some Juniperus L. species from the flora of Azerbaijan. TIMBB, 10 (1), 54-61.